Exploring nanopore direct sequencing performance of forensic STRs, SNPs, InDels, and DNA methylation markers in a single assay
Our exploratory study using ONT adaptive sequencing for conventional forensic and age associated DNA methylation markers showed high sequencing accuracy for a significant number of markers, showcasing ONT as a promising (epi)genetic forensic method. Future studies must address three critical aspects: determining clear quantity and quality measures and detection thresholds for accuracy, optimizing input DNA quantity for forensic casework expectations, and addressing ethical considerations associated with phenotype and ancestry analysis to prevent ethnic biases.
Authors
- Jerry Hoogenboom
- Titia Sijen (ORCID: https://orcid.org/0000-0001-5944-7235)
- Yaran Zhang (ORCID: https://orcid.org/0000-0003-2601-0824)
- Amade M’charek (ORCID: https://orcid.org/0000-0001-5242-2406)
- Henne Holstege (ORCID: https://orcid.org/0000-0002-7688-3087)
- Marcel Reinders (ORCID: https://orcid.org/0000-0002-1148-1562)
- Peter Henneman (ORCID: https://orcid.org/0000-0003-2179-7808)
- Alex Salazar (ORCID: https://orcid.org/0000-0002-8453-8769)
- Kristiaan J. van der Gaag (ORCID: https://orcid.org/0000-0001-7976-1977)
- Natalie Weiler (ORCID: https://orcid.org/0000-0002-0745-2873)
- Martin A. Haagmans (ORCID: https://orcid.org/0009-0007-9283-9696)
- Niccoló Tesi (ORCID: https://orcid.org/0000-0002-1413-5091)
- Desiree D S H de Bruin
Institutions
- Netherlands Forensic Institute (NL)
- Amsterdam Neuroscience (NL)
- Amsterdam University Medical Centers (NL)
- Amsterdam University of Applied Sciences (NL)
- Vrije Universiteit Amsterdam (NL)
- University of Amsterdam (NL)
- Delft University of Technology (NL)
Publication Details
- Journal
- Forensic Science International Genetics
- Published
- 2024-10-12
- DOI
- https://doi.org/10.1016/j.fsigen.2024.103154
- Citations
- 10
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 2.34