The effect of different cytokinin and auxin source on micropropagation of Kalanchoe blossfeldiana

Micropropagation is widely used for vegetative propagation of many different ornamental and medicinal plant species under aseptic conditions. This technology has widespread practical applications irrespective of seasonal and time-space constraints. The present study aimed to design in vitro propagation using 1 8 different combinations of BAP, NAA, TDZ, and GA3 supplemented with commercial tea sugar at 30 g l-1 for micropropagation using leaf segments close to petiole of Kalanchoe blossfeldiana as explants and to find its efficiency for viable cultures. The results showed clear discrimination between regeneration on TDZ and BAP-supplemented regeneration cultures. Thus, both BAP-NAA and TDZ-NAA combinations were found suitable for micropropagation. the source medium affected the shoot regeneration and the rooting ability, irrespective of the culture of any shoot regeneration medium. These plantlets were rooted in 0.01-0.05 mg l-1 IBA. The study showed successful rooting under in vitro conditions using 1 ½ or 1×MS medium with 0.5 mg L-1 IBA. These rooted plantlets were then transferred to MS medium with 0.2 mg L-1 gibberellic acid and without BAP or with 0.1, 0.2, 0.5 mg l-1 TDZ and 0.1 mg l-1 NAA or without 0.1 mg L-1 NAA for adaptation. The results of the study are of great importance for K. blossfeldiana plant breeding studies and growers.