3D spheroids versus 2D-cultured human adipose stem cells to generate smooth muscle cells in an internal anal sphincter-targeting cryoinjured mouse model
These findings demonstrate that, compared to 2D-cultured hASCs, 3D-spheroids more effectively induce a contractile phenotype that is initially weak but subsequently improves, inducing expression of RhoA/ROCKII-downstream molecules and SMC differentiation associated with IAS basal tone.
Authors
- Il Tae Son (ORCID: https://orcid.org/0000-0003-1781-9189)
- Bo Young Oh (ORCID: https://orcid.org/0000-0002-1255-0961)
- Sung‐Bum Kang (ORCID: https://orcid.org/0000-0002-9574-5069)
- Dogeon Yoon
- You-Rin Kim (ORCID: https://orcid.org/0000-0001-6922-7383)
- Wook Chun (ORCID: https://orcid.org/0000-0002-4915-1497)
- Minsung Kim (ORCID: https://orcid.org/0000-0002-1984-5084)
- Ji‐Seon Lee
- Ji Hye Park (ORCID: https://orcid.org/0000-0001-9782-9197)
Institutions
- Hallym University (KR)
- Seoul National University Bundang Hospital (KR)
- Sacred Heart Hospital (NG)
- Hallym University Hangang Sacred Heart Hospital (KR)
- Hallym University Sacred Heart Hospital (KR)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2024-10-12
- DOI
- https://doi.org/10.1186/s13287-024-03978-9
- Citations
- 3
- Primary Topic
- Pelvic floor disorders treatments
- Type
- article
- Field-Weighted Citation Impact
- 1.41
Funders
- National Research Foundation
- Ministry of Education
- National Research Foundation of Korea