A novel strategy for improving ammonia resistance of acidogenesis using domesticated sludge combined with nZVI addition in an ambient anaerobic digestion system
High ammonia stress, which inhibited the performance and stability of anaerobic digestion (AD) systems, is considered a bottleneck problem. To improve the performance of ambient acidogenic AD system under high ammonia stress, three different strategies were developed, including native sludge with nano zero valent iron (nZVI) addition (S<sub>nZVI</sub>), domesticated sludge enriched with homoacetogens with no additive (S<sub>Domesticated</sub>) and domesticated sludge with nZVI addition (S<sub>Domesticated+nZVI</sub>). All groups were operated at ambient temperature (24 ± 1 °C). Results showed that ammonia stress restricted the acidogenic rate in ambient acidogenic system significantly. Under ammonia stress, both S<sub>Domesticated</sub> and S<sub>Domesticated+nZVI</sub> showed positive impact on acidogenesis to resist, while the nZVI solely of S<sub>nZVI</sub> couldn't relieve the ammonia stress effectively. Compared to nZVI or domesticated sludge solely added system, S<sub>Domesticated+nZVI</sub> showed highest acidogenic rate under high ammonia stress. The S<sub>Domesticated</sub> treatment increased acetic acid and ethanol production under high ammonia stress compared to the S<sub>Control</sub>. The S<sub>Domesticated+nZVI</sub> further increased the production of formic acid and H<sub>2</sub> and reduced the generation of CO<sub>2</sub>. Microbial community analysis indicated that the relative abundances of main acidogens Bifidobacterium, Solobacterium and ethanol producing bacteria Ethanoligenens, increased in the S<sub>Domesticated</sub> and S<sub>Domesticated+nZVI</sub> groups. Moreover, S<sub>Domesticated+nZVI</sub> increased the relative abundance of relevant functional enzyme-encoding genes involved in the generation of acetic acid, formic acid and ethanol and reduced the relative abundance of key functional enzyme-encoding genes related to butyric acid production. This work could provide a novel practical strategy to improve the performance of ambient acidogenic AD system under ammonia stress.
Authors
- Zhi‐Long Ye
- Nan Lv (ORCID: https://orcid.org/0000-0001-8105-8877)
- Guanjing Cai (ORCID: https://orcid.org/0000-0003-4736-5595)
- Dongping Xie (ORCID: https://orcid.org/0000-0003-1225-1088)
- Xiaofang Pan (ORCID: https://orcid.org/0000-0003-1289-389X)
- Yanlin Li (ORCID: https://orcid.org/0000-0002-4715-9030)
- Zunjing Lv
Institutions
- University of Science and Technology of China (CN)
- Institute of Urban Environment (CN)
- CAS Key Laboratory of Urban Pollutant Conversion (CN)
Publication Details
- Journal
- Water Research
- Published
- 2024-10-12
- DOI
- https://doi.org/10.1016/j.watres.2024.122619
- Citations
- 9
- Primary Topic
- Anaerobic Digestion and Biogas Production
- Type
- article
- Field-Weighted Citation Impact
- 2.39
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- Fujian Provincial Department of Science and Technology
- Institute of Urban Environment, Chinese Academy of Sciences