Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohort
We compared different antineutrophil cytoplasmic antibody (ANCA) detection methods using a predominantly myeloperoxidase (MPO)-ANCA-associated vasculitis cohort. Stored sera from 147 patients with untreated ANCA-associated vasculitis (AAV), including microscopic polyangiitis and granulomatosis with polyangiitis (<i>n</i> = 115 and 32, respectively), and 124 disease controls were tested for P-ANCA and C-ANCA with immunofluorescence (IIF), and for MPO-ANCA and proteinase 3 (PR3)-ANCA with different antigen-specific immunoassays: direct enzyme-linked immunosorbent assay (ELISA), chemiluminescent enzyme immunoassay (CLEIA), third-generation fluorescent enzyme immunoassay (FEIA), and latex turbidimetrical immunoassay (LTIA). In addition, MPO-ANCA and PR3-ANCA titers were calibrated using certified reference materials (CRMs). The sensitivities and specificities for AAV diagnoses were 95% and 94% (IIF), 86% and 98% (ELISA), 93% and 94% (CLEIA), 92% and 96% (FEIA), and 68% and 88% (LTIA). Dual IIF/antigen-specific immunoassay testing reduced diagnostic accuracies from 94% to 93%. The quantitative agreement between ANCA levels measured using CLEIA and FEIA and calibrated using CRMs was not good. In conclusion, this study demonstrated the high performance of antigen-specific immunoassays for AAV diagnosis in a predominantly MPO-ANCA-associated vasculitis cohort and suggested that the benefit of dual IIF/antigen-specific immunoassay testing is limited. Standardizing ANCA measurements using different immunoassays was difficult, even when using CRMs.
Authors
- Noriyuki Homma
- Daijo Inaguma (ORCID: https://orcid.org/0000-0002-3977-5933)
- Takao Saito (ORCID: https://orcid.org/0000-0002-1279-6400)
- Akihiro Ishizu (ORCID: https://orcid.org/0000-0002-7084-9734)
- Taichi Hayashi
- Yoshinori Matsumoto (ORCID: https://orcid.org/0000-0001-7047-2976)
- Masayoshi Harigai (ORCID: https://orcid.org/0000-0002-6418-2603)
- Izaya Nakaya (ORCID: https://orcid.org/0000-0002-0792-6514)
- Tsuyoshi Watanabe (ORCID: https://orcid.org/0000-0002-3528-4782)
- Shinya Kaname (ORCID: https://orcid.org/0000-0002-9507-180X)
- Hitoshi Sugiyama (ORCID: https://orcid.org/0000-0002-4127-6471)
- Kazuhiko Tsuruya (ORCID: https://orcid.org/0000-0002-8390-2928)
- Hiroaki Dobashi (ORCID: https://orcid.org/0000-0003-2944-5583)
- Kosaku Nitta (ORCID: https://orcid.org/0000-0002-9548-054X)
- Eri Muso (ORCID: https://orcid.org/0000-0002-1150-7419)
- Naotake Tsuboi (ORCID: https://orcid.org/0000-0003-0760-845X)
- Sakae Homma (ORCID: https://orcid.org/0000-0002-6213-3584)
- Satoshi Horikoshi (ORCID: https://orcid.org/0000-0001-6588-5821)
- Taio Naniwa (ORCID: https://orcid.org/0000-0002-0489-2611)
- Ken‐ei Sada (ORCID: https://orcid.org/0000-0003-1020-0818)
- Shin‐ichi Takeda (ORCID: https://orcid.org/0000-0003-0152-1150)
- Yohko Murakawa (ORCID: https://orcid.org/0000-0002-7122-4832)
- Shogo Banno (ORCID: https://orcid.org/0000-0002-4376-5770)
- Yasuhiro Katsumata (ORCID: https://orcid.org/0000-0003-1654-0119)
- Nobuhito Hirawa (ORCID: https://orcid.org/0000-0001-5824-3652)
- Naoto Tamura (ORCID: https://orcid.org/0000-0003-1729-2954)
- Atsushi Komatsuda (ORCID: https://orcid.org/0000-0003-1477-8131)
- Takahiko Sugihara (ORCID: https://orcid.org/0000-0002-7181-7494)
- Shoichi Fujimoto
- Junichi Hirahashi (ORCID: https://orcid.org/0000-0003-3881-2322)
- Takashi Wada
- Noriyoshi Ogawa (ORCID: https://orcid.org/0009-0003-9470-0904)
- Naoki Nakagawa (ORCID: https://orcid.org/0000-0002-5398-3667)
- Tatsuya Atsumi (ORCID: https://orcid.org/0000-0001-5657-962X)
- Kunihiro Yamagata (ORCID: https://orcid.org/0000-0002-7407-0410)
- Masayuki Iwano (ORCID: https://orcid.org/0000-0002-3044-4679)
- Tsutomu Takeuchi (ORCID: https://orcid.org/0000-0003-1111-8218)
- Koichi Amano (ORCID: https://orcid.org/0000-0002-4228-2056)
- Masaaki Nakano
- Satoshi Ito (ORCID: https://orcid.org/0000-0002-8464-7535)
- Hirofumi Makino (ORCID: https://orcid.org/0000-0002-1559-3799)
- Atsushi Kawakami (ORCID: https://orcid.org/0000-0002-1512-8782)
- Hitoshi Hasegawa (ORCID: https://orcid.org/0000-0003-3902-2066)
- Yasushi Kawaguchi (ORCID: https://orcid.org/0000-0003-2025-1344)
- Tomohiro Kameda (ORCID: https://orcid.org/0000-0003-4521-008X)
- Takao Fujii
- Shoichi Ozaki
- Yukio Yuzawa
- Masaharu Yoshida
- Masahiro Yamamura
- Makoto Ogura
- Ritsuko Katafuchi
- Takafumi Ito
Institutions
- Kanazawa University (JP)
- University of Fukui (JP)
- University of Miyazaki (JP)
- Toho University (JP)
- Fukushima Medical University (JP)
- Kyushu University (JP)
- Fujita Health University (JP)
- University of Tsukuba (JP)
- Asahikawa Medical University (JP)
- Okayama University (JP)
- Health Sciences University of Hokkaido (JP)
- St. Marianna University School of Medicine (JP)
- Aichi Medical University (JP)
- Shimane University (JP)
- Kyorin University (JP)
- Kagawa University (JP)
- Akita University (JP)
- Keio University (JP)
- Hokkaido University (JP)
- Kyoto University (JP)
- Yokohama City University Medical Center (JP)
- Hamamatsu University School of Medicine (JP)
- Fukuoka University (JP)
- Nagoya City University (JP)
- Juntendo University (JP)
- Kochi Medical School Hospital (JP)
- Niigata Prefectural Shibata Hospital (JP)
- Iwate Prefectural Central Hospital (JP)
- Takamatsu Red Cross Hospital (JP)
- Tokyo Medical University Hachioji Medical Center (JP)
- Toho University Omori Medical Center (JP)
- Okayama University Hospital (JP)
- Fukuoka Higashi Medical Center (JP)
- Tokyo Women's Medical University (JP)
- Jikei University Kashiwa hospital (JP)
- Okayama Saiseikai General Hospital (JP)
- Tokyo Metropolitan Geriatric Hospital (JP)
- Japanese Red Cross Nagoya Daini Hospital (JP)
- Social Insurance Saitama Chuo Hospital (JP)
- Center for Rheumatology (US)
- Kushiro City General Hospital (JP)
- Kitano Hospital (JP)
- Ehime University (JP)
- Nagasaki University (JP)
- Niigata University (JP)
- The University of Tokyo (JP)
- Saitama Medical University (JP)
- Yokohama City University (JP)
Publication Details
- Journal
- Immunological Medicine
- Published
- 2024-10-11
- DOI
- https://doi.org/10.1080/25785826.2024.2408054
- Citations
- 1
- Primary Topic
- Vasculitis and related conditions
- Type
- article
- Field-Weighted Citation Impact
- 0.42