Single-shot 20-fold expansion microscopy

Abstract Expansion microscopy (ExM) is in increasingly widespread use throughout biology because its isotropic physical magnification enables nanoimaging on conventional microscopes. To date, ExM methods either expand specimens to a limited range (~4–10× linearly) or achieve larger expansion factors through iterating the expansion process a second time (~15–20× linearly). Here, we present an ExM protocol that achieves ~20× expansion (yielding <20-nm resolution on a conventional microscope) in a single expansion step, achieving the performance of iterative expansion with the simplicity of a single-shot protocol. This protocol, which we call 20ExM, supports postexpansion staining for brain tissue, which can facilitate biomolecular labeling. 20ExM may find utility in many areas of biological investigation requiring high-resolution imaging.

Authors

Institutions

Publication Details

Journal
Nature Methods
Published
2024-10-11
DOI
https://doi.org/10.1038/s41592-024-02454-9
Citations
31
Primary Topic
Advanced Fluorescence Microscopy Techniques
Type
article
Field-Weighted Citation Impact
20.53

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Single-shot 20-fold expansion microscopy

Wang Shiwei, Ryan B. McMillan, Kylie S. Leung, Peng Yin et al.
31 citations
Nature Methods
Advanced Fluorescence Microscopy Techniques
20.53
article

Single-shot 20-fold expansion microscopy

Wang Shiwei, Ryan B. McMillan, Kylie S. Leung, Peng Yin, Hanquan Su, Laura L. Kiessling, Tay Shin, Yixi Liu, Chi Zhang, Harley B. Yoder, Edward S. Boyden
article en
31 citations

Abstract

Abstract Expansion microscopy (ExM) is in increasingly widespread use throughout biology because its isotropic physical magnification enables nanoimaging on conventional microscopes. To date, ExM methods either expand specimens to a limited range (~4–10× linearly) or achieve larger expansion factors through iterating the expansion process a second time (~15–20× linearly). Here, we present an ExM protocol that achieves ~20× expansion (yielding <20-nm resolution on a conventional microscope) in a single expansion step, achieving the performance of iterative expansion with the simplicity of a single-shot protocol. This protocol, which we call 20ExM, supports postexpansion staining for brain tissue, which can facilitate biomolecular labeling. 20ExM may find utility in many areas of biological investigation requiring high-resolution imaging.

Nature MethodsVol. 21(11)
Broad Institute (US), Howard Hughes Medical Institute (US), Harvard University (US), McGovern Institute for Brain Research (US), Harvard University Press (US), Harvard Bioscience (United States) (US), Center for Systems Biology (US), Massachusetts Institute of Technology (US)
Howard Hughes Medical Institute, Office of Extramural Research, National Institutes of Health
Openalex Percentile: Top 1%
Advanced Fluorescence Microscopy Techniques
20.53
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.