Single-Shot 3D Imaging Meta-Microscope
Three-dimensional (3D) imaging enables high-precision and high-resolution axial positioning, which is crucial for biological imaging, semiconductor defect monitoring, and other applications. Conventional implementations rely on bulky optical elements or scanning mechanisms, resulting in low speed and complicated setups. Here, we generate the double-helix (DH) point spread function with an all-dielectric metasurface and thus innovate the 3D imaging microscope (hence dubbed meta-microscope), both in 4f and 2f imaging systems. The 4f-meta-microscope with a numerical aperture of 0.7 achieves an axial localization accuracy below 0.12 μm within a 15.47 μm detection range, while the 2f-DH meta-microscope with a numerical aperture of 0.3 shows a 1.12 μm accuracy within a 227.33 μm range. We also demonstrate single-shot and accurate 3D biological imaging of the mouse kidney tissue and peach anther, providing a comprehensive and efficient approach for 3D bioimaging and other applications through a single-shot 3D meta-microscope.
Authors
- Joel K. W. Yang (ORCID: https://orcid.org/0000-0003-3301-1040)
- Guangwei Hu (ORCID: https://orcid.org/0000-0002-3023-9632)
- Tong Ling (ORCID: https://orcid.org/0000-0001-9009-7966)
- M. A. Rahman (ORCID: https://orcid.org/0000-0003-3199-9251)
- Xumin Ding (ORCID: https://orcid.org/0000-0002-7725-790X)
- Shutao Zhang (ORCID: https://orcid.org/0000-0002-9343-5550)
- Jiubin Tan (ORCID: https://orcid.org/0000-0002-0941-7932)
- Xinwei Wang (ORCID: https://orcid.org/0009-0008-1718-0438)
- Haoyu Li (ORCID: https://orcid.org/0000-0002-0004-9336)
- Jian Liu (ORCID: https://orcid.org/0000-0001-7732-1505)
- Hao Wang (ORCID: https://orcid.org/0000-0001-5388-6691)
- Wenlong Lu (ORCID: https://orcid.org/0000-0002-3998-2801)
- Chengfeng Pan (ORCID: https://orcid.org/0009-0003-5103-7196)
- Huijie Hao
Institutions
- Agency for Science, Technology and Research (SG)
- Singapore University of Technology and Design (SG)
- Nanyang Technological University (SG)
- Harbin Institute of Technology (CN)
- Singapore National Eye Center (SG)
- Singapore-HUJ Alliance for Research and Enterprise (SG)
- Singapore Eye Research Institute (SG)
- Institute of Materials Research and Engineering (SG)
- Huazhong University of Science and Technology (CN)
- Ministry of Industry and Information Technology (CN)
Publication Details
- Journal
- Nano Letters
- Published
- 2024-10-11
- DOI
- https://doi.org/10.1021/acs.nanolett.4c03952
- Citations
- 18
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 11.92
Funders
- National Research Foundation Singapore
- Ministry of Education - Singapore
- National Natural Science Foundation of China
- National Key Research and Development Program of China