Protocol for isolating antigen-specific monoclonal antibodies from immunized mice utilizing the Beacon platform
Isolation of antigen-specific plasma B cells had been challenging until the recent arrival of the Beacon platform. Leveraging light-sorting technology, Beacon can perform high-throughput screening of plasma B cells on a chip to sort single cells with the desired antigen specificity. Here, we present a protocol for isolating antigen-specific plasma B cells from immunized mice using Beacon, sequencing the encoded B cell receptors (BCRs), and cloning and expressing the resulting antibodies. This protocol can easily be extended to human samples.
Authors
- Longfei Ding (ORCID: https://orcid.org/0000-0002-7660-0876)
- Kangli Cao
- Shubing Tang (ORCID: https://orcid.org/0000-0001-6639-0222)
- Shimeng Bai (ORCID: https://orcid.org/0000-0003-4020-1934)
- Xiaoyan Zhang (ORCID: https://orcid.org/0000-0002-3193-1401)
- Jianqing Xu
- Meilan Fu
- Xiangwei Wang (ORCID: https://orcid.org/0009-0005-6582-2480)
- Chen Zhao (ORCID: https://orcid.org/0000-0002-0718-7707)
- Yangyang Hu (ORCID: https://orcid.org/0009-0001-1412-9185)
- Tianhan Yang
Institutions
- Fudan University (CN)
- Shanghai Public Health Clinical Center (CN)
- Zhongshan Hospital (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-10-11
- DOI
- https://doi.org/10.1016/j.xpro.2024.103389
- Citations
- 1
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.40
Funders
- National Natural Science Foundation of China
- Science and Technology Commission of Shanghai Municipality