Quantitative Biodistribution of OMVs Using SPECT/CT Imaging with HYNIC-Duramycin Radiolabeling
<i>Introduction:</i> Bacterial outer membrane vesicles (OMVs) are emerging as important players in the host-microbiome interaction, while also proving to be a promising platform for vaccine development and targeted drug delivery. The available methods for measuring their biodistribution, however, are limited. We aimed to establish a high-efficiency radiolabeling method for the treatment of OMVs. <i>Methods:</i> <sup>99m</sup>Tc-HYNIC-duramycin was incubated with OMVs isolated from <i>E. coli</i> BL21(DE3) ΔnlpI ΔlpxM. Radiolabeling efficiency (RLE) and radiochemical purity (RCP) were measured with size-exclusion high-performance liquid chromatography. The biodistribution was quantitatively measured in mice using SPECT/CT imaging. <i>Results:</i> RLE was 81.84 ± 2.03% for undiluted OMV suspension and 56.17 ± 2.29% for 100× dilution. Postlabeling purification with a spin-desalting column results in 100% radioactivity in the OMV fraction according to HPLC, indicating 100% RCP of the final product. The biodistribution was found to be in line with previous data reported in the literature using other OMV tracking attempts. <i>Conclusions:</i> Our findings illustrate that using HYNIC-duramycin for labeling of the OMVs enhances efficiency and is easily implementable for in vivo imaging studies, significantly improving upon earlier methods.
Authors
- Judith Mihály (ORCID: https://orcid.org/0000-0003-4291-3345)
- Dávid Szöllősi (ORCID: https://orcid.org/0000-0002-3363-3862)
- Kanni Das Shailaja
- Krisztián Szigeti (ORCID: https://orcid.org/0000-0002-0828-9828)
- Anikó Gaál (ORCID: https://orcid.org/0000-0003-4064-1825)
- Tamás Felföldi (ORCID: https://orcid.org/0000-0003-2009-2478)
- Domokos Máthé (ORCID: https://orcid.org/0000-0001-7343-0413)
- Balázs Zoltán Gulyás (ORCID: https://orcid.org/0000-0001-9295-2460)
- Polett Hajdrik (ORCID: https://orcid.org/0000-0002-5452-4892)
- Hedvig Tordai (ORCID: https://orcid.org/0000-0002-0875-5569)
- Bálint Jezsó (ORCID: https://orcid.org/0000-0002-1306-4797)
- Zoltán V. Varga (ORCID: https://orcid.org/0000-0002-2758-0784)
- Ralf Bergmann (ORCID: https://orcid.org/0000-0002-8733-4286)
- Ildikó Horváth (ORCID: https://orcid.org/0000-0002-9390-7925)
- Parasuraman Padmanabhan (ORCID: https://orcid.org/0000-0003-4112-4600)
Institutions
- Semmelweis University (HU)
- Eötvös Loránd University (HU)
- Nanyang Technological University (SG)
- Budapest University of Technology and Economics (HU)
- Institute of Materials and Environmental Chemistry (HU)
- HUN-REN Centre for Ecological Research (HU)
- Montavid Thermodynamic Research Group (HU)
- Institute of Molecular Life Sciences (HU)
- HUN-REN Research Centre for Natural Sciences (HU)
Publication Details
- Journal
- ACS Omega
- Published
- 2024-10-11
- DOI
- https://doi.org/10.1021/acsomega.4c04632
- Citations
- 1
- Primary Topic
- Bacterial Infections and Vaccines
- Type
- article
- Field-Weighted Citation Impact
- 0.34
Funders
- Budapesti Műszaki és Gazdaságtudományi Egyetem
- Nanyang Technological University
- Semmelweis Egyetem
- Magyar Tudományos Akadémia
- Eötvös Loránd Tudományegyetem
- Nemzeti Kutatási Fejlesztési és Innovációs Hivatal
- Horizon 2020 Framework Programme
- Lee Kong Chian School of Medicine, Nanyang Technological University