In-vivo tissue healing mechanism at the intestinal anastomosis site following high-frequency electric welding

To investigate the effect of high-frequency electric welding (HFEW) on intestinal tissue healing, we performed end-to-end anastomosis experiments in New Zealand rabbits. Within one week post-surgery, animals exhibited normal vital signs, replaced necrotic tissue with healthy collagen, and showed improved tissue strength while inflammation decreased. By day 60, tissue pathology and function fully recovered, resembling normal tissue. Healing at the anastomotic site occurred in three phases: immediate adhesion, inflammation, and remodeling, with macrophages crucial for phagocytosis and regeneration of necrotic tissue. This study enhances understanding of HFEW's healing mechanisms and supports further preclinical investigations.