Evaluation of the Effects of a Neurotransmitter on the Action Potential Parameters in Lumbricus Terrestris

Background of the Study: Action potentials (APs) are crucial for transmitting electrical signals in neurons. They are influenced by the balance of ions across the membrane and the permeability of sodium and potassium channels. Acetylcholine (ACh) affects neural activity, but its specific impact on AP parameters needs to be understood, mainly when administered near the ventral nerve area. Aim: This study investigated the effects of acetylcholine on action potential parameters in L. terrestris, focusing on changes in response time, peak point, valley point, and wave width of AP evoked by electrical stimulation. Methodology: The L. terrestris were placed on electrode arrays of the data acquisition system to record APs in their ventral nerve under 90 mV electrical stimulation. Baseline recordings were taken, followed by the administration of ACh near the ventral nerve cord. The resulting changes in AP parameters were analyzed to assess the impact of these substances on neural conduction. Conclusions: Acetylcholine increased the response time and peak amplitude of action potentials, while wave width and valley point were not dependent on the amount of Ach volume injected. Further clinical or in-vitro studies might be needed to improve our system understanding.