Research on the Effects of the <i>Juncus Inflexus</i> Subsp. <i>Inflexus</i> Extract on Low Dose Thioacetamide‐Induced Liver Damage: A Novel Antioxidant

Abstract In this study was investigated the effects of extract of the Juncus inflexus subsp. inflexus ( JI) plant on thioacetimide (TAA)‐induced liver damage. Six experimental groups were formed using 42 8–12‐weeks old Sprague dawley male rats, with seven experimental animals per group. Liver tissue sections were examined by staining with hematoxylin eosin (H‐E), periodic acid schiff (PAS) and caspase‐3. On the other hand, the tissue homogenates were analyzed by enzyme linked immunosorbent assay (ELISA), in terms of malondialdehyde (MDA) and glutathione (GSH). Compared to other groups, it was observed that the MDA rose significantly in the TAA group and that the GSH fell and that the expression of the caspase‐3 was significantly increased. On the other hand, it was found that lipid peroxidation decreased significantly in the TAA + JI group when compared to JI + TAA group. Also, to see the antioxidant properties of the plant in detail, molecular dynamic simulations were done. The antioxidant property of 59.16% in extract compounds of JI was theoretically demonstrated. The antioxidant activity of the 10‐methoxy‐nb‐alpha‐methylcorynantheol molecule, which is found at the highest rate in the relevant plant, has been theoretically revealed for the first time. In conclusion, this study has revealed that the extraction obtained from the Juncus inflexus plant has protective and therapeutic properties for the liver.