A Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to Quantify Wine Polyphenols

Polyphenols are responsible for wine colour and astringency, and, as antioxidants, they also have beneficial health properties. In this work, we developed a robust full-scan high-resolution mass spectrometry method for the quantification of 90 phenolic compounds in wine samples (either red, rosé, or white wine), using a UHPLC-Orbitrap<sup>TM</sup> system. With this method, we could conduct a detailed analysis of phenolic compounds in red, rosé, and white wines with great selectivity due to sub-ppm mass accuracy. Moreover, accessing the full-scan spectrum enabled us to monitor all the other compounds detected in the sample, facilitating the adaptability of this method to new phenolic compounds if needed.

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Publication Details

Journal
Methods and Protocols
Published
2024-10-10
DOI
https://doi.org/10.3390/mps7050082
Citations
2
Primary Topic
Metabolomics and Mass Spectrometry Studies
Type
article
Field-Weighted Citation Impact
0.47

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article

A Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to Quantify Wine Polyphenols

Nicolas Sommerer, Emmanuelle Meudec, Aécio Luís de Sousa Dias, Damien Flores
2 citations
Methods and Protocols
Metabolomics and Mass Spectrometry Studies
0.47
article

A Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to Quantify Wine Polyphenols

Nicolas Sommerer, Emmanuelle Meudec, Aécio Luís de Sousa Dias, Damien Flores
article en
2 citations

Abstract

Polyphenols are responsible for wine colour and astringency, and, as antioxidants, they also have beneficial health properties. In this work, we developed a robust full-scan high-resolution mass spectrometry method for the quantification of 90 phenolic compounds in wine samples (either red, rosé, or white wine), using a UHPLC-Orbitrap<sup>TM</sup> system. With this method, we could conduct a detailed analysis of phenolic compounds in red, rosé, and white wines with great selectivity due to sub-ppm mass accuracy. Moreover, accessing the full-scan spectrum enabled us to monitor all the other compounds detected in the sample, facilitating the adaptability of this method to new phenolic compounds if needed.

Methods and ProtocolsVol. 7(5)
Université de Montpellier (FR), Institut Agro Montpellier (FR), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), Institut Agro Montpelier (FR), L'Institut Agro (FR)
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Openalex Percentile: Top 35%
Metabolomics and Mass Spectrometry Studies
0.47
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