Live-Cell-Imaging-Analysen mit Crumbs2-Varianten

Abstract Crumbs2 (CRB2) is a novel, less-studied key component of the renal filtration barrier and part of the slit diaphragm formed by podocytes. Some CRB2 variants have been linked to inherited forms of proteinuric diseases from childhood. However, the pathogenic potential of many CRB2 variants is still unknown. Here we report the development of a simple but robust live-cell imaging-based system, allowing a first evaluation about the pathogenic potential of novel CRB2 variants.