Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes
Extracellular production can reduce the cost of recombinant proteins by simplifying downstream processing. In the case of the PET-hydrolysing cutinase ICCG<sub>DAQI</sub>, it was even possible to avoid chromatographic purification and still achieve efficient PET hydrolysis. With such production approaches and their further optimisation, enzymatic recycling of PET can contribute to a more efficient and environmentally friendly solution to the industrial recycling of plastics in the future.
Authors
- Kathrin Castiglione (ORCID: https://orcid.org/0000-0001-9086-0036)
- Marco Oldiges (ORCID: https://orcid.org/0000-0003-0704-5597)
- Stefanie Fritzsche (ORCID: https://orcid.org/0000-0001-7882-5211)
- Holger Hübner
Institutions
- Forschungszentrum Jülich (DE)
- Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
- RWTH Aachen University (DE)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2024-10-10
- DOI
- https://doi.org/10.1186/s12934-024-02547-2
- Citations
- 10
- Primary Topic
- Microplastics and Plastic Pollution
- Type
- article
- Field-Weighted Citation Impact
- 1.33
Funders
- Friedrich-Alexander-Universität Erlangen-Nürnberg