Next generation sequencing identifies an increased diversity of microbes in post lavage specimens in infected TKA using a biofilm disrupting irrigant
In this pre-to-post irrigant study, culture showed a decrease in the number of identifiable organisms post-lavage. In contrast NGS revealed an increase in the number of identifiable organisms post-lavage. Furthermore, NGS identified 135 additional organisms, not detected pre-lavage. This suggests an increased diversity of microbes may exist within EMPM, which are not cultivable.
Authors
- Madhav Chowdhry (ORCID: https://orcid.org/0000-0003-0769-0326)
- Alexandra I. Stavrakis (ORCID: https://orcid.org/0000-0002-2391-7650)
- Matthew V. Dipane (ORCID: https://orcid.org/0000-0001-5978-8662)
- Edward J. McPherson (ORCID: https://orcid.org/0000-0003-0327-4181)
- Diego García (ORCID: https://orcid.org/0000-0002-5047-6846)
- Stephen T Duncan
Institutions
- University of Kentucky (US)
- University of California, Los Angeles (US)
- Nuffield Health (GB)
Publication Details
- Journal
- The Knee
- Published
- 2024-10-10
- DOI
- https://doi.org/10.1016/j.knee.2024.09.010
- Citations
- 5
- Primary Topic
- Infectious Diseases and Tuberculosis
- Type
- article
- Field-Weighted Citation Impact
- 2.86