A Closer Look at N1-methylpseudouridine in the Modified mRNA Injectables

Introducing 728 N1-methylpseudouridines into the spike coding sequence for SARS-CoV-2 has inevitably resulted in physical changes in the original SARS-CoV-2 coding sequence. These non-negligible physical changes include: (1) stereochemical alterations; (2) variations in molecular weight; and (3) changes in the nucleotide base count consisting of A, G, T(U, Ψ), C. Assuming only that things are going as planned by the inventors of the new technology, the physical changes in each of the 728 substitutions in the SARS-CoV-2 spike coding sequence, where a uridine is replaced with an N1-methylpseudouridine, engages the ribosome as it reads, interprets, and translates the modified mRNA spike coding sequence into a specific sequence of amino acids. Whatever the peptide/protein sequence turns out to be must set up a cascading series of downstream consequences from whatever adjustments occur in the largely unpredictable peptide/protein sequences being produced by the ribosome. The stereoscopic changes in moving from uridine to pseudouridine involve rotating the uracil ring structure 180º and shifting three carbon positions clockwise; then, the further modification of pseudouridine to N1-methylpseudouridine involves the introduction of a methyl group leading to even more noticeable changes in the stereochemical configuration. I also document physical changes in molecular weight and in base count. Such physical modifications must have cascading effects on interactions across all levels of the downstream peptide/protein products produced from the never before encountered sequences of nucleotides. Given such physical modifications, can the proteinaceous materials produced by the modified mRNA coding sequences lead to the production of effective antibodies against the SARS-CoV-2 spike protein? What can be expected from repeated exposures to these foreign modified mRNA sequences through multiple doses and subsequent booster injections? Empirical outcomes, it seems, get worse with each new dose rather than better.