Equine coronavirus infection and replication in equine intestinal enteroids
In this study, equine intestinal enteroids (EIEs) were generated from the duodenum, jejunum, and ileum and inoculated with equine coronavirus (ECoV) to investigate their suitability as in vitro models with which to study ECoV infection. Immunohistochemistry revealed that the EIEs were composed of various cell types expressed in vivo in the intestinal epithelium. Quantitative reverse-transcription PCR (qRT-PCR) and virus titration showed that ECoV had infected and replicated in the EIEs. These results were corroborated by electron microscopy. This study suggests that EIEs can be novel in vitro tools for studying the interaction between equine intestinal epithelium and ECoV.
Authors
- Manabu Nemoto (ORCID: https://orcid.org/0000-0002-6154-2263)
- Daiki Kishi
- Akihiro Ochi (ORCID: https://orcid.org/0000-0002-8972-3090)
- Nanako Kawanishi
- Koji Tsujimura (ORCID: https://orcid.org/0009-0001-8198-2514)
- Hiroshi Bannai (ORCID: https://orcid.org/0000-0002-9573-5901)
- Minoru Ohta (ORCID: https://orcid.org/0000-0001-8131-5044)
- Takanori Ueno (ORCID: https://orcid.org/0000-0003-0973-2710)
- Yoshinori Kambayashi (ORCID: https://orcid.org/0000-0002-3033-8489)
- Tohru Suzuki (ORCID: https://orcid.org/0000-0002-3607-4787)
Institutions
- National Institute of Animal Health (JP)
- Japan Racing Association (JP)
Publication Details
- Journal
- Veterinary Research
- Published
- 2024-10-10
- DOI
- https://doi.org/10.1186/s13567-024-01381-z
- Citations
- 1
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.39
Funders
- Japan Racing Association