Legacy and emergent contaminants in glaucous-winged gull eggs from Canada's Pacific coast: Spatial distribution, temporal trends, and risks for human consumers

Using glaucous-winged gull (Larus glaucescens) eggs from Canada's Pacific coast, we investigated spatial and temporal trends (2008-2022) of a suite of legacy and emergent contaminants, including 16 perfluoroalkyl substances (PFAS), 15 polybrominated diphenyl ethers (PBDEs), 7 alternative halogenated flame retardants (AHFRs), total mercury (THg), as well as stable isotopes of carbon (δ¹³C) and nitrogen (δ¹⁵N) to control for diet. Legacy organochlorines (OCs) were also measured in eggs in 2020 for a preliminary human health risk assessment (HHRA). Between 2008 and 2022, glaucous-winged gull eggs from more urban-influenced colonies (Mandarte Island) were up to ∼2x more contaminated with PFAS, PBDEs, AHFRs, and THg than eggs from the offshore colony (Cleland Island), suggesting different source regions and dietary exposures. Concentrations of Σ₁₅PBDEs declined linearly among colonies (p < 0.001), consistent with several North American phase-outs and regulatory restrictions dating back to the early/mid 2000s. Conversely, temporal trends for PFOS, Σ₁₂PFCAs, Σ₇AHFRs, and THg were characterized by a combination of second-order declines and non-linear increases in recent years. After correcting THg for dietary shifts using δ¹⁵N, THg concentrations followed a U-shaped trend at Mandarte and Cleland Islands, while those at Mitlenatch Island remained relatively constant over time. Increasing trends for some contaminants coincided with both an increase in δ¹³C and δ¹⁵N. For the HHRA, all gull eggs collected in 2020 had hazard quotients (HQs) < 0.2, indicating no foreseeable risk or harm for First Nations consumers for certain contaminants. Our findings indicate that spatio-temporal trends of persistent contaminants in Pacific glaucous-winged gull eggs are influenced by a combination of factors, including the impact of regulations on anthropogenic emissions, coupled with changes in foraging behaviour and food-web structure.