Systematic perturbations of SETD2, NSD1, NSD2, NSD3, and ASH1L reveal their distinct contributions to H3K36 methylation
Within genes, SETD2 primarily deposits H3K36me3, while the other K36MTs deposit H3K36me1/2 independently of SETD2 activity. For the deposition of H3K36me1/2, we find a hierarchy of K36MT activities where NSD1 > NSD2 > NSD3 > ASH1L. While NSD1 and NSD2 are responsible for most genome-wide propagation of H3K36me2, the activities of NSD3 and ASH1L are confined to active regulatory elements.
Authors
- Francisca N. de Luna Vitorino (ORCID: https://orcid.org/0000-0001-8543-2299)
- Reinnier Padilla (ORCID: https://orcid.org/0009-0005-5646-5450)
- Eric Bareke (ORCID: https://orcid.org/0000-0003-3928-7160)
- Jacek Majewski (ORCID: https://orcid.org/0000-0003-3572-9558)
- Bo Hu (ORCID: https://orcid.org/0000-0001-8527-3750)
- Cynthia Horth (ORCID: https://orcid.org/0000-0003-2441-6783)
- Chao Lü (ORCID: https://orcid.org/0000-0003-0982-8122)
- Gerry A. Shipman
- Benjamin Aaron Garcia (ORCID: https://orcid.org/0000-0002-2306-1207)
- Joanna M. Gongora (ORCID: https://orcid.org/0009-0006-7613-8219)
Institutions
- Washington University in St. Louis (US)
- Columbia University Irving Medical Center (US)
- McGill Genome Centre (CA)
- McGill University (CA)
Publication Details
- Journal
- Genome biology
- Published
- 2024-10-10
- DOI
- https://doi.org/10.1186/s13059-024-03415-3
- Citations
- 16
- Primary Topic
- Epigenetics and DNA Methylation
- Type
- article
- Field-Weighted Citation Impact
- 3.51
Funders
- McGill University