Histotripsy-Induced Bactericidal Activity Correlates to Size of Cavitation Cloud In Vitro

Large abscesses are walled-off collections of pus and bacteria that often do not respond to antibiotic therapy. Standard of care involves percutaneous placement of indwelling catheter(s) for drainage, a long and uncomfortable process with high rehospitalization rates. The long-term goal of this work is to develop therapeutic ultrasound approaches to eradicate bacteria within abscesses as a noninvasive therapeutic alternative. Inertial cavitation induced by short pulses of focused ultrasound (histotripsy) is known to generate lethal mechanical damage in bacteria. Prior studies with Escherichia coli (E. coli) in suspension demonstrated that bactericidal effects increase with increasing peak negative amplitude, treatment time, and duty cycle. The current study investigated correlates of bactericidal activity with histotripsy cavitation cloud size. Histotripsy was applied to E. coli suspensions in 10-mL sample vials at 810 kHz, 1.2 MHz, or 3.25 MHz for 40 min. The cavitation activity in the sample vials was separately observed with high-speed photography. The cavitation cloud area was quantified from those images. A linear relationship was observed between bacterial inactivation and cavitation cloud size ( ), regardless of the acoustic parameters (specifically frequency, pulse duration, and power) used to produce the cloud.Index Terms- Abscess, bacterial inactivation, bactericidal activity, cavitation, high intensity focused ultrasound (HIFU), histotripsy, therapeutic ultrasound.

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Journal
IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control
Published
2024-10-09
DOI
https://doi.org/10.1109/tuffc.2024.3476438
Primary Topic
Spectroscopy Techniques in Biomedical and Chemical Research
Type
article
Field-Weighted Citation Impact
0.00

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article

Histotripsy-Induced Bactericidal Activity Correlates to Size of Cavitation Cloud In Vitro

Keith Chan, Gilles Thomas, Wayne L. Monsky, Matthew Bruce et al.
IEEE Transactions on Ultrasonics Ferroelectrics and Frequency Control
Spectroscopy Techniques in Biomedical and Chemical Research
article

Histotripsy-Induced Bactericidal Activity Correlates to Size of Cavitation Cloud In Vitro

Keith Chan, Gilles Thomas, Wayne L. Monsky, Matthew Bruce, Yak-Nam Wang, Adam D. Maxwell, Thomas J. Matula, W. Conrad Liles, Yashwanth Nanda Kumar, E. Patchen Dellinger, Stephanie Totten, Daniel F. Leotta, Tatiana D. Khokhlova, Jeff Thiel, Pratik A. Ambekar, Pavel B. Rosnitskiy, Adeyinka Adedipe, Shelby Pierson, Kaizer Contreras
article en

Abstract

Large abscesses are walled-off collections of pus and bacteria that often do not respond to antibiotic therapy. Standard of care involves percutaneous placement of indwelling catheter(s) for drainage, a long and uncomfortable process with high rehospitalization rates. The long-term goal of this work is to develop therapeutic ultrasound approaches to eradicate bacteria within abscesses as a noninvasive therapeutic alternative. Inertial cavitation induced by short pulses of focused ultrasound (histotripsy) is known to generate lethal mechanical damage in bacteria. Prior studies with Escherichia coli (E. coli) in suspension demonstrated that bactericidal effects increase with increasing peak negative amplitude, treatment time, and duty cycle. The current study investigated correlates of bactericidal activity with histotripsy cavitation cloud size. Histotripsy was applied to E. coli suspensions in 10-mL sample vials at 810 kHz, 1.2 MHz, or 3.25 MHz for 40 min. The cavitation activity in the sample vials was separately observed with high-speed photography. The cavitation cloud area was quantified from those images. A linear relationship was observed between bacterial inactivation and cavitation cloud size ( ), regardless of the acoustic parameters (specifically frequency, pulse duration, and power) used to produce the cloud.Index Terms- Abscess, bacterial inactivation, bactericidal activity, cavitation, high intensity focused ultrasound (HIFU), histotripsy, therapeutic ultrasound.

IEEE Transactions on Ultrasonics Ferroelectrics and Frequency ControlVol. 71(12: Breaking the Resolution)
California Polytechnic State University (US), University of Washington (US), Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences (US), Vantage View (United States) (US), University of Washington Applied Physics Laboratory (US), Seattle University (US), Virginia Tech (US)
U.S. Department of Energy, Washington State University, University of Washington, National Institutes of Health, California State University, Fresno, National Institute of General Medical Sciences, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Biomedical Imaging and Bioengineering
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Openalex Percentile: Top 89%
Spectroscopy Techniques in Biomedical and Chemical Research
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