Bio-functionalized hydrogel patches of chitosan for the functional recovery of infarcted myocardial tissue
The aim of this work was to assess the potential benefits of the enrichment of a chitosan hydrogel patch with secretome and its epicardial implantation in a murine model of chronic ischemia, focusing on the potential to restore the functional capacity of the heart. Thus, a hydrogel with a final polymer concentration of 3 % was prepared from chitosan with an acetylation degree of 24 % and then bio-functionalized with a secretome produced by mesenchymal stromal cells. The identification of proteins in the secretomes showed the presence of several proteins known to have beneficial effects on cardiac muscle repair. Then chitosan hydrogels were immersed in secretome. The protein incorporation in the hydrogel and their release over time were studied, demonstrating the ability of the gel to retain and then deliver proteins (around 40 % was released in the first 6 h, and then a plateau was reached). Moreover, mechanical analysis exhibited that the patches remained suturable after enrichment. Finally, bio-functionalized hydrogel patches were sutured onto the surface of the infarcted myocardium in rat. Thirty days after, the presence of enriched hydrogels induced a reversion of cardiac function which seems to come mainly from an improvement of left ventricle systolic performance and contractility.
Authors
- Elodie Pillet (ORCID: https://orcid.org/0000-0002-2314-8548)
- M. Baraibar
- Loris Commin
- Orianne Domengé (ORCID: https://orcid.org/0000-0003-2395-3552)
- C. Bonnefont (ORCID: https://orcid.org/0000-0002-4059-4961)
- Alexandra Montembault (ORCID: https://orcid.org/0000-0003-1820-5832)
- Nathalie Mougenot (ORCID: https://orcid.org/0000-0002-7707-234X)
- Agnès Crépet (ORCID: https://orcid.org/0000-0002-1746-5846)
- N. Saulnier (ORCID: https://orcid.org/0000-0003-0159-8414)
- Onnik Agbulut (ORCID: https://orcid.org/0000-0003-1923-8871)
- Thierry Delair (ORCID: https://orcid.org/0000-0003-0983-4557)
- Robin Deloux (ORCID: https://orcid.org/0000-0002-4601-266X)
- Gaëlle Revet
- A. Cavagnino
- Alexandre Simon
- Laetitia Mazière (ORCID: https://orcid.org/0009-0007-6211-3800)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Sorbonne Université (FR)
- Centre d'Économie de la Sorbonne (FR)
- VetAgro Sup (FR)
- Institut de Biologie Paris-Seine (FR)
- Ingenierie des Materiaux polymeres (FR)
- Science et Médecine (France) (FR)
- Adaptation Biologique et Vieillissement (FR)
- UMS Phénotypage du petit animal (FR)
- Institut National des Sciences Appliquées de Lyon (FR)
Publication Details
- Journal
- International Journal of Biological Macromolecules
- Published
- 2024-10-09
- DOI
- https://doi.org/10.1016/j.ijbiomac.2024.136400
- Citations
- 3
- Primary Topic
- Tissue Engineering and Regenerative Medicine
- Type
- article
- Field-Weighted Citation Impact
- 1.72
Funders
- Fondation de l'Avenir pour la Recherche Médicale Appliquée
- Agence Nationale de la Recherche
- Institut National de la Santé et de la Recherche Médicale
- Fédération Française de Cardiologie
- Fondation de France
- Université de Lyon