OsKANADI1 and OsYABBY5 regulate rice plant height by targeting <i>GIBERELLIN 2-OXIDASE6</i>
Plant height is an important agronomic characteristic of rice (Oryza sativa L.). Map-based cloning analyses of a natural semi-dwarf rice mutant with inwardly curled leaves found in the field revealed that the defects were due to a mutation of a SHAQKYF-class MYB family transcription factor, OsKANADI1 (OsKAN1). OsKAN1 directly bound to the OsYABBY5 (OsYAB5) promoter to repress its expression and interacted with OsYAB5 to form a functional OsKAN1-OsYAB5 complex. GIBERELLIN 2-OXIDASE6 (OsGA2ox6), encoding an enzyme in the gibberellin (GA) catabolic pathway, was activated by OsYAB5. Furthermore, the OsKAN1-OsYAB5 complex suppressed the inhibitory effect of OsKAN1 toward OsYAB5 and inhibited OsYAB5-induced OsGA2ox6 expression. The proOsKAN1:OsYAB5 transgenic plants were taller than wild-type plants, whereas oskan1 proOsKAN1:OsYAB5 plants exhibited a severe dwarf phenotype due to the absence of the OsKAN1-OsYAB5 complex. The OsKAN1-OsYAB5 complex modulated OsGA2ox6 expression, thereby regulating the levels of bioactive gibberellins and, consequently, plant height. This study elucidated the mechanism underlying the effect of the OsKAN1-OsYAB5-OsGA2ox6 regulatory pathway on plant height at different positions in rice stems and provided insights on stem development and candidate genes for the aerial architecture improvement of crop plants.
Authors
- Yong Zhang (ORCID: https://orcid.org/0000-0003-3704-4835)
- Qinqin Han
- Wanqing Lv
- Hao Wu (ORCID: https://orcid.org/0000-0002-6752-7160)
- Li Wang (ORCID: https://orcid.org/0000-0001-7434-9385)
- Danning Wang (ORCID: https://orcid.org/0009-0007-1354-8015)
- Longjun Zeng (ORCID: https://orcid.org/0000-0002-1355-4761)
- Guosheng Xiong (ORCID: https://orcid.org/0000-0002-4312-1676)
- Quan Wang (ORCID: https://orcid.org/0000-0003-2912-1263)
- Xuelian Zheng (ORCID: https://orcid.org/0000-0002-7475-7888)
- Shuting Zhang
- Qi He (ORCID: https://orcid.org/0009-0003-8093-9455)
- Y. Tan (ORCID: https://orcid.org/0000-0001-8537-1182)
- Zhiqiang Liao (ORCID: https://orcid.org/0009-0001-3100-9687)
- Caiyun Yin (ORCID: https://orcid.org/0000-0001-9121-4212)
Institutions
- Nanjing Agricultural University (CN)
- Southwest University (CN)
- University of Electronic Science and Technology of China (CN)
- Henan University (CN)
- Shenzhen University (CN)
- Jiangsu Academy of Agricultural Sciences (CN)
- Agricultural Genomics Institute at Shenzhen (CN)
- Cotton Research Institute (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- Institute of Crop Sciences (CN)
Publication Details
- Journal
- The Plant Cell
- Published
- 2024-10-09
- DOI
- https://doi.org/10.1093/plcell/koae276
- Citations
- 13
- Primary Topic
- Oil Palm Production and Sustainability
- Type
- article
- Field-Weighted Citation Impact
- 5.97
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Chinese Academy of Agricultural Sciences
- Basic and Applied Basic Research Foundation of Guangdong Province