Comparative analysis of iPSC-derived NK cells from two differentiation strategies reveals distinct signatures and cytotoxic activities
Our work demonstrated that both protocols are capable of producing functional iNK cells. However, the two sets of resulting iNKs exhibited distinct phenotypes and transcriptomic profiles. The lymphoid-based differentiation approach generated iNKs with a more mature and activated profile, which demonstrated higher cytotoxicity against cancer cell lines compared to iNK cells produced under short-term feeder-free conditions suggesting that the differentiation strategy must be considered when designing iNK cell-based adoptive immunotherapies.
Authors
- Hudson Bezerra (ORCID: https://orcid.org/0000-0002-6072-5746)
- Diana Chaker (ORCID: https://orcid.org/0000-0003-2670-8341)
- Ali G. Turhan (ORCID: https://orcid.org/0000-0002-4861-0137)
- Frank Griscelli (ORCID: https://orcid.org/0000-0003-0318-2754)
- Jusuf Imeri (ORCID: https://orcid.org/0000-0002-1456-7388)
- Annelise Bennaceur‐Griscelli (ORCID: https://orcid.org/0000-0002-3557-4499)
- Christophe Desterke (ORCID: https://orcid.org/0000-0001-7679-2524)
- Nathan Belliard
- Matthias Huyghe
- Noufissa Oudrirhi
Institutions
- Inserm (FR)
- Université Paris Cité (FR)
- Université Paris-Saclay (FR)
- Institut Gustave Roussy (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Hôpital Paul-Brousse (FR)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2024-10-09
- DOI
- https://doi.org/10.3389/fimmu.2024.1463736
- Citations
- 6
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 1.44