On-Site Visualization Assay for Tumor-Associated miRNAs: Using Ru@TiO<sub>2</sub> as a Peroxidase-like Nanozyme
Accurate diagnosis of highly aggressive and deadly tumors is essential for effective treatment and improved patient outcomes, and microRNAs (miRNAs) have emerged as crucial biomarkers for their roles in tumor initiation, progression, and metastasis. Herein, we present an on-site visualization colorimetric assay for tumor-associated miRNAs using ruthenium nanoparticle decorated titanium dioxide nanoribbon (Ru@TiO<sub>2</sub>) as a peroxidase-like (POD) nanozyme. Remarkably, the Ru@TiO<sub>2</sub> nanozyme can catalyze the oxidation of chromogenic substrates through its POD-like activity, which is effectively inhibited by pyrophosphate generated during the rolling circle amplification process, thereby enabling miRNA detection through a visible colorimetric readout. This approach provides a highly sensitive and specificity assay for miRNAs in diluted human serum with a detection limit of 100 pM. It shows great potential for clinical diagnostics and biological research, offering a promising tool for early cancer diagnosis and molecular diagnostics.
Authors
- Jie Hu (ORCID: https://orcid.org/0000-0002-6137-9064)
- Walter Hu (ORCID: https://orcid.org/0000-0002-6748-1916)
- Xun He (ORCID: https://orcid.org/0000-0002-2525-9102)
- Yuchun Ren (ORCID: https://orcid.org/0009-0002-9375-0902)
- Hao Bai (ORCID: https://orcid.org/0000-0002-0916-5755)
- Weihua Zhuang (ORCID: https://orcid.org/0000-0003-0492-7527)
- Xuping Sun (ORCID: https://orcid.org/0000-0002-5326-3838)
- Yongchao Yao (ORCID: https://orcid.org/0000-0002-8210-7121)
- Binwu Ying (ORCID: https://orcid.org/0000-0001-8077-3908)
- Limei Zhang (ORCID: https://orcid.org/0000-0001-9522-790X)
- Fengming Luo (ORCID: https://orcid.org/0000-0001-9267-3437)
- Xingzhi Yu
- Zhengwei Cai
- Yongsong Luo (ORCID: https://orcid.org/0000-0002-0234-0477)
- Peilin Wu
Institutions
- University of Electronic Science and Technology of China (CN)
- Sichuan University (CN)
- Shandong Normal University (CN)
- West China Hospital of Sichuan University (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-10-09
- DOI
- https://doi.org/10.1021/acs.analchem.4c03922
- Citations
- 25
- Primary Topic
- Advanced Nanomaterials in Catalysis
- Type
- article
- Field-Weighted Citation Impact
- 2.93
Funders
- National Natural Science Foundation of China
- National Science and Technology Major Project
- Fundamental Research Funds for the Central Universities