Chemical analysis of Alliin-Rich Allium sativum (Garlic) extract and its safety evaluation in Drosophila melanogaster

Garlic (<i>Allium sativum</i>) has been traditionally valued for its medicinal properties attributed to the presence of organosulfur compounds. Despite its benefits, concerns about herbal extract toxicity have arisen, necessitating safety assessment <i><b>.</b></i> This study was designed to evaluate the chemical analysis and safety profile of Alliin-Rich Garlic Extract (ARGE) using <i>Drosophila melanogaster</i> as a model organism. The ARGE was extracted from garlic cloves (<i>Allium sativum</i> Linn: UIH-23262) using a microwave-assisted method and characterized using UPLC-ESI-MS, ¹H NMR, HPLC and IR. Its safety evaluation was determined using <i>D. melanogaster</i> (Harwich strain), and various assays were conducted on 1-3-day-old flies. Toxicological markers and oxidative stress were assessed to understand the impact of ARGE on the flies. Chemical profiling of ARGE using UPLC-ESI-MS, confirmed the presence of alliin (S-ally-L-cysteine-S-oxide), L-arginine, γ-glutamylmethionine, S-(2-carboxypropyl) glutathione, N-γ-glutamyl-S-(1-propenyl) cysteine, N-γ-glutamyl-S-(2-propenyl) cysteine, N-γ-glutamylphenylalanine, S-(allylthio) cysteine, γ-glutamyl-S-allylthiocysteine and eruboside B. HPLC confirmed an alliin content of 0.073 mg/g. Toxicological assessment in <i>D. melanogaster</i> revealed that ARGE enhanced antioxidant defenses by increasing total thiol levels and GST activity, while reducing acetylcholinesterase activity. No significant alteration was observed in catalase activity and cellular metabolic rate. Histological examination revealed no alterations in the histoarchitecture of the brain, fat body or gut of <i>D. melanogaster</i>. The study demonstrated the safety of ARGE in <i>D. melanogaster</i>, supporting its potential as a safe herbal remedy.