Neem Leaves Extract Reduces Sex Steroids and Gonadal Function in Female Wistar Albino Rats

The neem plant (Azadirachta indica) is one of the most important medicinal plants in Asia and Africa. This study aimed to investigate the possible anti-fertility potential of dietary neem leaves' extract in female Wistar albino rats as. This model could be further applied to stray dogs. In this experiment 24 adult female albino Wister rats were divided into two groups: control and treated with neem leave extract, they were tested for estrous regularity by vaginal smears, progesterone, and estrogen hormone were measured. Ovarian caspase-3 expression and histopathological inspection were examined. The results have revealed reducing sex steroids, increment in ovarian caspase-3 expression, and histological deteriorations in the ovary and uterus. The study concluded that neem flower extract can be used as an antifertility agent in animals.