Protein Film Electrochemistry and Spectroscopic Measurements of Transmembrane Metalloenzymes

Redox-active metalloproteins including metalloenzymes play important roles in denitrification, photosynthesis and respiration. To understand their enzymatic reaction mechanisms and the effects of reaction environments on protein properties, protein film electrochemistry (PFE) coupled with surface-enhanced infrared absorption (SEIRA) spectroscopy is a powerful technique. Herein, we present and discuss PFE–SEIRA spectroscopy results on the determination of reduction potential, formation processes of bilayer lipid membranes on the electrode surface, and effects of protein-protein or protein-lipid interactions on the enzymatic activities for transmembrane metalloenzymes of cytochrome c-dependent nitric oxidase and cytochrome c oxidase.