Enhancement of Osteoblastogenesis in the hFOB 1.19 Cell Line by the Induction of the n-Butanol Fraction Derived from Marsilea crenata C Presl. Leaves.

Osteoporosis is a disease characterized by bone loss, which can be treated by increasing the bone formation process. A marker of increased bone formation processes is the activity of the transcription factor Osx which stimulates the formation of Ocn. M. crenata leaves contains phytoestrogens which are thought to increase bone formation. The purpose of this research was to prove that the n-butanol fraction from the leaves of M. crenata can increase bone formation in hFOB 1.19 osteoblast cells by increasing the activity of the transcription factors Osx and Ocn. hFOB 1.19 cells that had reached confluence were given n-butanol fraction at a dose of 62.5, 125, and 250 µg/L, and Genistein 2.5 µg/ml as a positive control for phytoestrogens. Increased bone formation was identified through the expression of Osx and Ocn using the immunocytochemical method with CLSM. The findings indicate that the application of various dosages of the n-butanol fraction derived from the leaves of M. crenata has a positive impact on bone formation in hFOB 1.19 cells. The best dose was determined to be 250 µg/L, with a statistically significant difference of p<0.005. The n-butanol fraction obtained from M. crenata leaves was found to increase the expression of Osx and Ocn in hFOB 1.19 cells, indicating its potential to enhance bone production.