Protective effects of lemon nanovesicles: evidence of the Nrf2/HO-1 pathway contribution from in vitro hepatocytes and in vivo high-fat diet-fed rats
The cross-talk between plant-derived nanovesicles (PDNVs) and mammalian cells has been explored by several investigations, underlining the capability of these natural nanovesicles to regulate several molecular pathways. Additionally, PDNVs possess biological proprieties that make them applicable against pathological conditions, such as hepatic diseases. In this study we explored the antioxidant properties of lemon-derived nanovesicles, isolated at laboratory (LNVs) and industrial scale (iLNVs) in human healthy hepatocytes (THLE-2) and in metabolic syndrome induced by a high-fat diet (HFD) in the rat. Our findings demonstrate that in THLE-2 cells, LNVs and iLNVs decrease ROS production and upregulate the expression of antioxidant mediators, Nrf2 and HO-1. Furthermore, the in vivo assessment reveals that the oral administration of iLNVs improves glucose tolerance and lipid dysmetabolism, ameliorates biometric parameters and systemic redox homeostasis, and upregulates Nrf2/HO-1 signaling in HFD rat liver. Consequently, we believe LNVs/iLNVs might be a promising approach for managing hepatic and dysmetabolic disorders.
Authors
- Pierangelo Sardo (ORCID: https://orcid.org/0000-0002-4997-3263)
- Nima Rabienezhad Ganji (ORCID: https://orcid.org/0000-0001-9408-3721)
- Monica Frinchi (ORCID: https://orcid.org/0000-0002-1061-5725)
- Stefania Raimondo (ORCID: https://orcid.org/0000-0001-5413-1303)
- Giuseppe Vergilio (ORCID: https://orcid.org/0009-0002-8670-6356)
- Valentina Di Liberto (ORCID: https://orcid.org/0000-0002-8574-0061)
- Simona Fontana (ORCID: https://orcid.org/0000-0003-4681-2170)
- Danila Di Majo (ORCID: https://orcid.org/0000-0002-0533-6932)
- Giulia Urone (ORCID: https://orcid.org/0009-0000-3130-2202)
- Vincenza Tinnirello (ORCID: https://orcid.org/0009-0001-0715-370X)
- Alice Conigliaro (ORCID: https://orcid.org/0000-0002-3037-835X)
- Francesca Rappa (ORCID: https://orcid.org/0000-0001-6610-5268)
- Giuditta Gambino (ORCID: https://orcid.org/0000-0001-8155-2808)
- Roberta Gasparro (ORCID: https://orcid.org/0009-0008-2006-8885)
- Francesco Paolo Zummo (ORCID: https://orcid.org/0000-0001-9872-8574)
- Nicolò Ricciardi
- Giuseppe Ferraro (ORCID: https://orcid.org/0000-0003-0982-8114)
- Riccardo Alessandro (ORCID: https://orcid.org/0000-0002-9935-1040)
- Giulia Duca
Institutions
- Institute for Biomedical Research and Innovation (IT)
- University of Palermo (IT)
Publication Details
- Journal
- Biomedicine & Pharmacotherapy
- Published
- 2024-10-08
- DOI
- https://doi.org/10.1016/j.biopha.2024.117532
- Citations
- 5
- Primary Topic
- Genomics, phytochemicals, and oxidative stress
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- Università degli Studi di Palermo