A Direct Approach for Living Biomembrane Printing on a Nanoparticle

Biomembrane coating technologies have been developed to equip synthetic nanomaterials with natural biointerfaces. We report a one-step method for nondestructively coating the biomembranes of "living" cells onto nanoparticle surfaces. By using simple centrifugation, nanoparticles pass through a concentrated layer of living cells. This process mimics exosome release via endocytosis and exocytosis, preserving the membrane integrity of the source cells. The resulting silica nanoparticles were efficiently coated with membrane components from Raw264.7 cells. Nanoflow-liquid chromatography-tandem mass spectrometry confirmed that the proteins composing the membrane originated from the source cells. Additionally, the biomembrane coating suppressed the phagocytosis of silica nanoparticles by Raw264.7 cells while enhancing their uptake by HeLa cells. Our simple and efficient method for living biomembrane coating holds promise for the development of nanoparticles for medical and pharmaceutical applications.