Probing the Nanonewton Mitotic Cell Deformation Force by Ion-Resonance-Enhanced Photonics Force Microscopy
Mechanical forces are essential for regulating dynamic changes in cellular activities. A comprehensive understanding of these forces is imperative for unraveling fundamental mechanisms. Here, we develop a microprobe capable of facilitating the measurement of biological forces up to nanonewton levels in living cells. This probe is designed by coating the core of anatase titania particles with amorphous titania and silica shells and an upconversion nanoparticles (UCNPs) layer. Leveraging both antireflection and ion resonance effects from the shells, the optically trapped probe attains a maximum lateral optical trap stiffness of 14.24 pN μm<sup>-1</sup> mW<sup>-1</sup>, surpassing the best reported value by a factor of 3. Employing this advanced probe in a photonic force microscope, we determine the elasticity modulus of mitotic HeLa cells as 1.27 ± 0.3 kPa. Nanonewton probes offer the potential to explore 3D cellular mechanics with unparalleled precision and spatial resolution, fostering a deeper understanding of the underlying biomechanical mechanisms.
Authors
- Lei Ding (ORCID: https://orcid.org/0000-0002-9574-5165)
- Xuchen Shan (ORCID: https://orcid.org/0000-0001-7889-4006)
- Jianyun Wang (ORCID: https://orcid.org/0000-0001-9718-8633)
- Qian Peter Su (ORCID: https://orcid.org/0000-0001-7364-3945)
- Dejiang Wang (ORCID: https://orcid.org/0000-0001-5222-7037)
- Zhaoxiang Zhong (ORCID: https://orcid.org/0000-0002-0574-0185)
- Xiangjun Di
- Chaohao Chen (ORCID: https://orcid.org/0000-0003-4620-7771)
- Dajing Wang (ORCID: https://orcid.org/0000-0003-1637-6048)
- Zhiyong Song (ORCID: https://orcid.org/0000-0002-2552-5239)
- Min Zhang (ORCID: https://orcid.org/0000-0003-1412-3186)
- Shuhua Yue (ORCID: https://orcid.org/0000-0003-1830-008X)
- Fan Wang (ORCID: https://orcid.org/0000-0001-7403-3305)
- Faliang Cheng
Institutions
- University of Technology Sydney (AU)
- Australian National University (AU)
- Australian Research Council (AU)
- Pingdingshan University (CN)
- Dongguan University of Technology (CN)
- Yale University (US)
- Nanomaterials Research (United States) (US)
- Department of Physics, Mathematics and Informatics (BY)
- ARC Centre of Excellence for Transformative Meta-Optical Systems (AU)
- Beihang University (CN)
- Xi'an Jiaotong University (CN)
Publication Details
- Journal
- Nano Letters
- Published
- 2024-10-08
- DOI
- https://doi.org/10.1021/acs.nanolett.4c03610
- Citations
- 3
- Primary Topic
- Force Microscopy Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.01
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Beijing Municipal Natural Science Foundation