Multiplexed Random Access Approach to DNA Microspheres for High‐Capacity Data Storage

Abstract With the rapid growth of modern information data, DNA has emerged as a novel medium for data storage due to its durability, replicability, sustainability, and high density. This study focuses on the compatibility, high capacity, and random access of DNA data storage systems. A calcium phosphate (CaP) mineralized fluorescent SiO 2 DNA (FSD@CaP) microspheres is developed by encapsulating fluorescence‐labeled encoding DNA files onto the surface of the SiO 2 microspheres that are intrinsically sized and fluorescent as addressed two‐dimensional (2D) barcodes. The various sizes of SiO 2 and various fluorescence‐labeled encoding DNA files forming 2D barcodes of FSD@CaP. The 2D barcodes enhance the system's compatibility while allowing multiplexed random access to DNA files. The high‐capacity DNA storage permits effective single access to the DNA file using a minimal number of FSD@CaP microspheres, without impacting the matrix of the system. Ultimately, random access to arbitrary DNA files within a complex pool of distinct 2D barcode‐tagged FSD@CaP microspheres is demonstrated by utilizing a flow cytometer for multiplexed sorting. Consequently, the strategy of this storage system provides a scalable concept for compatibility and multiplexed random access to DNA data sets.