Anti-Cancer Effects of the <i>Pandanus tectorius</i> Parkinson Extract: Reduction of YAP and TAZ Levels via Inhibition of the Hippo and Notch Signaling Pathways

In this study, we aimed to investigate the anti-cancer effects of the Pandanus tectorius extract on AGS cells.P. tectorius, commonly known as hala or screw pine, is a tropical plant traditionally used for its medicinal properties, including antiinflammatory and antioxidant properties.Here, effects of the P. tectorius extract on cell proliferation, migration, and gene expression were evaluated using various assays, including the water-soluble tetrazolium salt (WST)-1, wound healing, migration, and western blotting assays.WST-1 assay revealed a significant dose-and time-dependent decrease in cell viability, with higher concentrations of the extract resulting in more pronounced viability inhibition.Wound healing and migration assays revealed that the P. tectorius extract effectively hindered cell migration, as the treated cells showed considerably slower wound closure and reduced migration than the control cells.Molecular analysis revealed that the extract significantly downregulated the expression levels of key oncogenic proteins, genes, and components of the Notch signaling pathway.Western blotting confirmed the substantial reduction in the marker protein levels in treated cells.These findings suggest that P. tectorius extract exerts its anti-cancer effects by inhibiting multiple signaling pathways crucial for cancer cell proliferation, migration, and survival.Overall, this study highlights the potential of P. tectorius extract as a therapeutic agent for gastric cancer treatment.