In vivo perturb-seq of cancer and microenvironment cells dissects oncologic drivers and radiotherapy responses in glioblastoma
In sum, we demonstrate the utility of multiplexed perturb-seq for in vivo single-cell dissection of adult cancer and normal tissue biology across multiple cell types in the context of therapeutic intervention, a platform with potential for broad application.
Authors
- Mitchel Stuart Berger (ORCID: https://orcid.org/0000-0003-1983-4892)
- Christopher Zou
- David Ronan Raleigh (ORCID: https://orcid.org/0000-0001-9299-8864)
- Thomas O’Loughlin (ORCID: https://orcid.org/0000-0002-6333-3991)
- David Wu (ORCID: https://orcid.org/0000-0003-1965-025X)
- Luke A. Gilbert (ORCID: https://orcid.org/0000-0001-5854-0825)
- Tomoko Ozawa
- Timothy Casey-Clyde
- Daniel A. Lim (ORCID: https://orcid.org/0000-0001-7221-3425)
- Ashir A. Borah (ORCID: https://orcid.org/0000-0003-1193-4987)
- William A. Weiss (ORCID: https://orcid.org/0000-0003-2230-9132)
- Roarke A. Kamber (ORCID: https://orcid.org/0000-0001-8462-4782)
- Joanna Pak
- Siyuan Liu (ORCID: https://orcid.org/0000-0003-1042-0191)
- A. P. Morse
- Dillon Pang
- Kyounghee Seo
Institutions
- University of California, San Francisco (US)
- American Research Center in Egypt (US)
- University of California San Francisco Medical Center (US)
- Palo Alto Institute (US)
- Neurological Surgery (US)
- UCSF Helen Diller Family Comprehensive Cancer Center (US)
- Arc Research Institute
- University of San Francisco (US)
- Icahn School of Medicine at Mount Sinai (US)
Publication Details
- Journal
- Genome biology
- Published
- 2024-10-07
- DOI
- https://doi.org/10.1186/s13059-024-03404-6
- Citations
- 14
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 3.27