Designing a multi-epitope construct using immuno-informatic tools to prepare a messenger RNA vaccine against Rhipicephalus microplus ticks
More than 1000 epitopes were predicted using immune informatic tools, and only high-scoring epitopes were selected. A multi-epitope construct was designed using bio-informatic tools, and its physicochemical properties were predicted. The design construct was inserted into the pVAX1 plasmid, and <i>in vitro</i> transcription was performed to prepare the mRNA. LNPs of mRNA were prepared and characterized to be used as vaccines. It was found that LNPs were stable and nanometer-sized.
Authors
- Kamran Ashraf (ORCID: https://orcid.org/0000-0002-5673-2310)
- Ubaid Ullah (ORCID: https://orcid.org/0000-0002-4379-3755)
- Wasim Shehzad (ORCID: https://orcid.org/0000-0001-5836-8412)
- Imran Rashid (ORCID: https://orcid.org/0000-0002-0280-9335)
Institutions
- University of Veterinary and Animal Sciences (PK)
Publication Details
- Journal
- Veterinary World
- Published
- 2024-10-01
- DOI
- https://doi.org/10.14202/vetworld.2024.2235-2247
- Citations
- 4
- Primary Topic
- vaccines and immunoinformatics approaches
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Higher Education Commision, Pakistan
- University of Veterinary and Animal Sciences