Generation of a Peptide Library from human Bone Marrow for the discovery and identification of novel bio active bone-marrow derived peptides

Generation of a Peptide Library from human Bone Marrow for the discovery and identification of novel bio active bone-marrow derived peptidesHuman bone marrow contains an abundance of proteins and peptides that are essential for the formation of blood cells.Thus, bone marrow is expected to contain a wealth of factors that influence cell growth, differentiation, and function.Aiming to identify novel bioactive peptides in human bone marrow, we established a method to generate a peptide library from primary human material.To this end, 25 kilograms of human bone (femur) from donors were collected, and the bone marrow was extracted.The eluted peptides were separated into 285 fractions, and consecutively tested for bioactivity e.g.immune modulation, cell viability and growth, and anti-viral activity.After further iterative bioassay guided sub-fractionation, peptides responsible for the function can be identified.In summary, our data show that human bone marrow contains peptides that modulate autophagy and inhibit viral infections.This project provides the basis for establishing peptide extraction methods from complex human tissues to identify novel peptide modulators of cellular processes.