Efficient and glycoeconomic synthesis of densely clustered mucin glycopeptides

Mucins are known for fully O-glycosylated multiple tandem repeats (TRs), like the PTTSTTS motif found in MUC5AC, which has ~240 copies and 6 glycosylation sites per TR.The lack of methods to access long, glyco-defined multi-TRs limits our understanding of how mucin glycosylation impacts its functional properties.While solid-phase synthesis (SPS) is a viable approach, its disadvantage is the requirement of excess amounts of costly and potentially delicate glycoamino acids.We strive to improve access to glycoamino acid building blocks and develop methods allowing glycoeconomic synthesis of densely clustered mucin glycopeptides.