An all-in-one automated peptide purification and synthesis solution

TLQIRGRERFEMYRELNEALELK #6 ID Crude IH-SPPS Flow-SPPS + HPLC IH-SPPS + PEC IH-SPPS + PEC + HPLC ID SequenceRapid parallel production of high-quality synthetic peptide sequences by solid-phase peptide synthesis (SPPS) and subsequent purification is a challenging yet essential pursuit.Neoantigen peptides, in particular, with medium to long lengths used in cancer immunotherapies need to be manufactured rapidly and with high purity.However, conventional batchsynthesis is slow and purification with sequential high-pressure liquid chromatography (HPLC) offers low throughput, leading to extended production times and potential product loss requiring repeated synthesis.Shorter and more reliable production timelines are essential to ensure the rapid delivery of life saving personalized medicine to patients.This study presents a PurePep solution to improve neoantigen production for cancer therapy.The integrated approach includes induction heating (IH) for rapid automated peptide synthesis followed by automated orthogonal PurePep EasyClean (PEC ) purification on the PurePep Chorus synthesizer.