Hyperspectral Imaging Combined with Machine Learning Can Be Used for Rapid and Non-Destructive Monitoring of Residual Nitrite in Emulsified Pork Sausages

The non-destructive and rapid monitoring system for residual nitrite content in processed meat products is critical for ensuring food safety and regulatory compliance. This study was performed to investigate the application of hyperspectral imaging in combination with machine learning algorithms to predict and monitor residual nitrite concentrations in emulsified pork sausages. The emulsified pork sausage was formulated with 1.5% (<i>w</i>/<i>w</i>) sodium chloride, 0.3% (<i>w</i>/<i>w</i>) sodium tripolyphosphate, 0.5% (<i>w</i>/<i>w</i>) ascorbic acid, and sodium nitrite at concentrations of 0, 30, 60, 90, 120, and 150 mg/kg, based on total sample weight. Hyperspectral imaging measurements were conducted by capturing images of the cross-sections and lateral sides of sausage samples in a linescan mode, covering the spectral range of 1000-2500 nm. The analysis revealed that higher nitrite concentrations could influence the protein matrix and hydrogen-bonding capacities, which might cause increased reflectance at approximately 1080 nm and 1280 nm. Machine learning models, including XGBoost, CATboost, and LightGBM, were employed to analyze the hyperspectral data. XGBoost demonstrated the best performance, achieving an <i>R</i>² of 0.999 and a root mean squared error of 0.095, highlighting its high predictive accuracy. This integration of hyperspectral imaging with advanced machine learning algorithms offers a non-destructive and real-time method for monitoring residual nitrite content in processed meat products, noticeably improving quality control processes in the meat industry. Additionally, real-time implementation in industrial settings could further streamline quality control and enhance operational efficiency. Further research should focus on validating these findings with larger sample sizes and more diverse datasets to ensure robustness.