The impact of the physicochemical properties of calcium phosphate ceramics on biocompatibility and osteogenic differentiation of mesenchymal stem cells
We demonstrate a reduction in solubility for all analyzed composite after the treatment with H<sub>2</sub>O and H<sub>2</sub>O<sub>2</sub>, accompanied by an enhancement in adsorption activity. This modification also resulted in an increase in micro- and macroporosity, along with a rise in the open porosity. Adipose-derived mesenchymal stromal cells demonstrated excellent cell adhesion and survival when cultured with these ceramics. Calcium phosphate ceramics (H-500, HT-500, and HT-1 series) stimulated alkaline phosphatase expression, promoted calcium deposition, and enhanced osteopontin expression in ADSCs, independently inducing osteogenesis without additional osteogenic stimuli. These findings underscore the promising potential of HAP-based bioceramics for bone regeneration/reconstruction.
Authors
- О. O. Piven (ORCID: https://orcid.org/0000-0002-6468-649X)
- S. A. Firstov (ORCID: https://orcid.org/0000-0003-1961-7708)
- L. L. Маcewicz (ORCID: https://orcid.org/0000-0001-8306-260X)
- Volodymyr Kolomiiets (ORCID: https://orcid.org/0000-0003-2322-7091)
- Volodymyr V. Balatskyi (ORCID: https://orcid.org/0000-0002-9893-3049)
- Nataliia Ulianchych
- Yuliia Skliarenko
- Yuliia Galuza
- O. S. Koryak
- Т. П. Рубан
Institutions
- National Academy of Sciences of Ukraine (UA)
- Instytut Biologii Doświadczalnej im. Marcelego Nenckiego (PL)
- Frantsevich Institute for Problems in Materials Science (UA)
- Institute of Molecular Biology and Genetics (UA)
- Technische Universität Dresden (DE)
- University of Tübingen (DE)
- Polish Academy of Sciences (PL)
Publication Details
- Journal
- BMC Research Notes
- Published
- 2024-10-07
- DOI
- https://doi.org/10.1186/s13104-024-06937-y
- Citations
- 6
- Primary Topic
- Bone Tissue Engineering Materials
- Type
- article
- Field-Weighted Citation Impact
- 1.11
Funders
- National Academy of Sciences of Ukraine