Simultaneous estimation of catechin hydrate and vanillic acid by HPTLC from Alternanthera sessilis plant extract and evaluating their pharmacological activity

Objective: The aim of this study was to identify and quantify catechin hydrate and vanillic acid from plant Alternanthera sessile and determine their antimicrobial and antioxidant activity. Method: By using toluene: ethyl acetate: formic acid (5:5:1v/v) as a mobile phase combination, separation of catechin hydrate and vanillic acid were carried out at 254 nm. Parameters validation of developed method were performed for linearity, precision, robustness, LOD and LOQ. Microorganism’s zone of inhibition determine by using agar well diffusion method and antimicrobial activity of these two phytoconstituents were evaluated. DPPH assay method was used for determination of antioxidant activity. Result: The Rf values for catechin hydrate and vanillic acid were found to be 0.49 and 0.75 respectively and validation of parameters showed linear and accurate method for analysis. The zone of inhibition was found immensely significant for S.aureus, B.subtillis and C. albicans which when compared to standard antimicrobial agents and gave promising results. Catechin hydrate and vanillic acid also shows significant antioxidant activity. Conclusion: The optimised method was found to be efficient, precise, accurate, specific and economic. Therefore, the method would be useful for qualitative and quantitative routine analysis in pharmaceutical industry. Also, the antimicrobial and antioxidant effect of these two phytochemicals shows excellent result which is useful in many pharmaceuticals and disease conditions.