Mosquito Tissue Ultrastructure-Expansion Microscopy (MoTissU-ExM) enables ultrastructural and anatomical analysis of malaria parasites and their mosquito
We discuss potential use cases for MoTissU-ExM for study of malaria parasite biology, and more broadly. We detail drawbacks or challenges MoTissU-ExM and imaging these expanded tissues, along with information troubleshooting this technique. Finally, we discuss how MoTissU-ExM could be applied and adapted in future to increase its utility.
Authors
- Sabrina Absalon (ORCID: https://orcid.org/0000-0003-2468-8156)
- Joel Vega-Rodríguez (ORCID: https://orcid.org/0000-0002-9576-0058)
- Benjamin Liffner (ORCID: https://orcid.org/0000-0002-1573-6139)
- Thiago Luiz Alves e Silva (ORCID: https://orcid.org/0000-0002-8520-5405)
Institutions
- National Institute of Malariology, Parasitology and Entomology (VN)
- Vector Oncology (United States) (US)
- Indiana University School of Medicine
- Indiana University – Purdue University Indianapolis (US)
Publication Details
- Journal
- BMC Methods
- Published
- 2024-10-07
- DOI
- https://doi.org/10.1186/s44330-024-00013-4
- Citations
- 14
- Primary Topic
- Amphibian and Reptile Biology
- Type
- article
- Field-Weighted Citation Impact
- 3.96
Funders
- American Heart Association
- University of New South Wales
- National Institutes of Health
- School of Medicine, Indiana University
- National Institute of Allergy and Infectious Diseases