The ABCD_AF291 antibody gives strong and specific signal in ultra-expansion microscopy analysis of HA tagged proteins in African trypanosomes
African trypanosomes expressing a flagellar central pair protein tagged with a triple hemagglutinin (HA) epitope in tandem repeat were analyzed by ultra-expansion microscopy (U-ExM) using the recombinant anti-HA antibody ABCD_AF291. ABCD_AF291 gave a strong and specific signal, demonstrating that it can be used to localize tagged proteins in U-ExM experiments.
Authors
- Athina Paterou
- Samuel Dean (ORCID: https://orcid.org/0000-0002-4792-2198)
- Shambhawee Neupane
- Julia Sáez Conde
Institutions
- University of Warwick (GB)
Publication Details
- Journal
- Antibody Reports
- Published
- 2024-10-07
- DOI
- https://doi.org/10.24450/journals/abrep.2024.e1625
- Citations
- 1
- Primary Topic
- Trypanosoma species research and implications
- Type
- article
- Field-Weighted Citation Impact
- 0.39
Funders
- University of Warwick
- Akademie Věd České Republiky
- Medical Research Council
- Biotechnology and Biological Sciences Research Council
- Warwick Medical School