Isolation, Extraction of Oil and Production of Biodiesel from Microalgae

This work is aimed at producing biodiesel from microalgae using Bold Basal Medium. After a month of cultivation, isolation, and harvesting, the microalgae were processed (oil was extracted using a chemical extraction procedure including alcohol with a 96% alcohol content and n-hexane). Transesterification was employed to produce the biodiesel from the extracted algal oil, and the resulting fuel was characterized. The results show that Chlorella sp, Desmid sp and Ankistrodesmus sp were isolated, density, viscosity, acid value, saponification, free fatty acid, peroxide value, iodine value, impurity level, and moisture content of Chlorella sp, Desmid sp and Ankistrodesmus sp oil were (0.805, 0.702, 0.716), (4.92, 4.45, 4.22), (1.51, 1.61, 1.62), (179.8, 178.61, 186.60), (0.86, 0.87, 0.88), (28.6, 28.9, 28.8), (0.31, 0.35, 0.33), (0.90, 0.80, 0.88) and (0.30, 0.36, 0.24) respectively, biodiesel had cetane number (126,125,127), density (0.79, 0.88, 0.87), flash point (126, 127, 125), calorific value (38.39, 38.68, 38.72), and viscosity (2.43, 2.66, 2.33) respectively, the quantity of biodiesel produced varied across all the replicates as the mean difference were less than the standard mean of 2.074. The production of biodiesel is practically possible in Nigeria, and the government should set up companies so as to diversify the oil sector and reduce pollution. The major limitation in this research work was using the traditional method for the isolation and identification of dominant microalgae instead of the molecular method.