8287 Maternal Factors Associated with Oxidative Stress in Newborns of Women with Gestational Diabetes Mellitus

Abstract Disclosure: A. Valdez Gonzalez: None. R.A. Gomez-Diaz: None. M. Ruiz: None. S. Ángeles Mejía: None. M. Díaz-Flores: None. E. González Carranza: None. M. Ramos Cervantes: None. L. Ramirez Garcia: None. M. Diaz Velazquez: None. G. Acevedo Rodriguez: None. N. Wacher: None. Introduction: In gestational diabetes mellitus (GDM), oxidative stress is increased. However, the maternal factors that are linked to elevated oxidative stress markers in neonates, in addition to GDM, and their impact on fetal programming are still unclear and warrant further study. Objective: To evaluate maternal factors associated with elevated oxidative stress markers in newborns of mothers with and without GDM. Material and Methods Design: prospective cohort. Women with and without GDM in the third trimester of pregnancy were included. Oxidative stress markers [malondialdehyde (MDA), reduced glutathione (GSH), antioxidant capacity (measured by DPPH)], lipid profile, were measured in maternal serum at the end of pregnancy and umbilical cord blood. Maternal and newborn anthropometric data were recorded. Information on food intake was collected using a food frequency questionnaire in the third trimester of pregnancy. Statistical analysis: Variables were analyzed with Mann-Whitney U and multiple linear regression using the SPSS program. Values of p<0.05 were considered statistically significant. Results: A total of 220 pairs were included, 119 with GDM. MDA concentrations in females with GDM were lower vs females without GDM (12.85 vs 15.56, p=0.001) while in newborns from mothers with GDM antioxidant capacity was higher vs. newborns without GDM (32.59 vs 23.58, p=0.002). There was a positive correlation between the newborns and their mothers in GSH (R2 0.511, p>0.001) and antioxidant capacity (R2 0.289, p<0.001); in addition, maternal MDA correlated with newborn GSH (R2 0.264, p>0.001). Maternal factors predicting newborn antioxidant capacity were: GDM (β 6.981, p=0.003), weight gain during pregnancy (β -0.461, p=0.024), maternal MDA (β -0.337, p=0.001) and antioxidant capacity (β 0.279, p<0.001), fruit (β 3.035, p=0.036) and vegetable consumption (β 4.098, p=0.014). For GSH, the associated factors were weight gain during pregnancy (β -0.124, p=0.019), pre-pregnancy BMI (β -0.161, p=0.024), maternal GSH (β 0.525, p<0.001) and cholesterol at the end of pregnancy (β -0.014, p=0.014); and for MDA levels were: GDM (β 4.607, p=0.039), pre-pregnancy overweight/obesity (β 5.130, p=0.036), maternal MDA (β 0.226, p=0.028) and cholesterol at the end of pregnancy (β -0.045, p=0.046). Conclusion: Maternal factors associated with the presence of oxidative stress markers in newborns were GDM, pre-pregnancy overweight/obesity and weight gain during gestation. In addition, fruit and vegetable consumption during pregnancy associate with increased antioxidant capacity. Presentation: 6/2/2024