NMR assignment of the conserved bacterial DNA replication protein DnaA domain IV
Chromosomal replication is a ubiquitous and essential cellular process. In bacteria, the master replication initiator DnaA plays a key role in promoting an open complex at the origin (oriC) and recruiting helicase in a tightly regulated process. The C-terminal domain IV specifically recognises consensus sequences of double-stranded DNA in oriC, termed DnaA-boxes, thereby facilitating the initial engagement of DnaA to oriC. Here, we report the <sup>13</sup>Cβ and backbone <sup>1</sup>H, <sup>15</sup>N, and <sup>13</sup>C chemical shift assignments of soluble DnaA domain IV from Bacillus subtilis at pH 7.6 and 298 K.
Authors
- Julia Hubbard (ORCID: https://orcid.org/0000-0001-8967-7154)
- Geoff Kelly (ORCID: https://orcid.org/0000-0002-1149-0717)
- Alexander Nguyen Abrams (ORCID: https://orcid.org/0009-0001-3562-402X)
Institutions
- The Francis Crick Institute (GB)
- Newcastle University (GB)
- Medical Research Council (GB)
Publication Details
- Journal
- Biomolecular NMR Assignments
- Published
- 2024-10-04
- DOI
- https://doi.org/10.1007/s12104-024-10206-1
- Primary Topic
- DNA Repair Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Wellcome Trust
- Cancer Research UK
- Medical Research Council