Microarray-based evaluation of selected recombinant timothy grass allergens expressed in E. Coli and N. Benthamiana
Recombinant allergens from both E. coli and N. benthamiana demonstrated strong diagnostic potential on the microarray platform, with Phl p 11 (MBP-fused) showing particularly high performance. High intra-assay variation highlights the need for further optimization in allergen formulation and microarray storage conditions. These results highlight the potential of recombinant allergens for diagnostic applications, despite challenges with allergen stability in microarray formats. Specific IgE prevalence to timothy allergens revealed a sensitization profile consistent with findings from multiple studies.
Authors
- Laimis Silimavičius (ORCID: https://orcid.org/0009-0005-8368-8350)
- Mindaugas Zaveckas
- Rasa Petraitytė-Burneikienė (ORCID: https://orcid.org/0000-0001-5676-2993)
- Kotryna Linauskienė (ORCID: https://orcid.org/0000-0002-4693-0436)
- Linas Griguola
- Laima Cepulyte (ORCID: https://orcid.org/0009-0003-7483-2694)
- Lieve Tchebotarev (ORCID: https://orcid.org/0009-0007-4435-0001)
- Raimundas Ražanskas (ORCID: https://orcid.org/0000-0003-3397-1797)
- Indrė Kučinskaitė-Kodzė (ORCID: https://orcid.org/0000-0002-1761-0089)
- Karolina Bielskė (ORCID: https://orcid.org/0009-0000-3768-9401)
Institutions
- Vilnius Gediminas Technical University (LT)
- Vilnius University (LT)
- Cytodiagnostics (Canada) (CA)
- Vilnius University Hospital Santariskiu Klinikos (LT)
Publication Details
- Journal
- BMC Biotechnology
- Published
- 2024-10-04
- DOI
- https://doi.org/10.1186/s12896-024-00902-0
- Citations
- 3
- Primary Topic
- Transgenic Plants and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.16
Funders
- European Regional Development Fund