Production of α-ketoisovalerate with whey powder by systemic metabolic engineering of Klebsiella oxytoca
The process introduced in this study enabled efficient α-ketoisovalerate production from low-cost substrate whey powder. Since the key genes for α-ketoisovalerate generation were integrated in genome of K. oxytoca KIV-7 and constitutively expressed, this strain is promising in stable α-ketoisovalerate fermentation and can be used as a chassis strain for α-ketoisovalerate derivatives production.
Authors
- Xiaoxu Tan
- Zhaoqi Kang
- Chuanjuan Lü (ORCID: https://orcid.org/0000-0003-4682-6089)
- Weikang Sun
- Cuiqing Ma (ORCID: https://orcid.org/0000-0002-7468-1027)
- Wensi Meng
- Chao Gao (ORCID: https://orcid.org/0000-0002-5205-0670)
- Tianyi Jiang
- Hui Zhang (ORCID: https://orcid.org/0009-0006-7530-3892)
- Shuo Wang (ORCID: https://orcid.org/0000-0002-7990-7515)
- Ping Xu (ORCID: https://orcid.org/0000-0002-4418-9680)
- Yidong Liu (ORCID: https://orcid.org/0000-0002-4631-0771)
- Leilei Guo
- Wenjia Tian
- Wei Liu (ORCID: https://orcid.org/0009-0001-6324-3830)
Institutions
- Shandong University (CN)
- Shanghai Jiao Tong University (CN)
- Qingdao Binhai University (CN)
- State Key Laboratory of Microbial Technology
- Shandong Jianzhu University (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2024-10-05
- DOI
- https://doi.org/10.1186/s12934-024-02545-4
- Citations
- 2
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Shandong University
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Natural Science Foundation of Shandong Province
- Natural Science Foundation of Qingdao