Proteomic analysis of storage proteins in Phaseolus vulgaris associated with resistance to water stress

The common bean ( Phaseolus vulgaris ), a widely consumed legume in Ecuador, boasts low economic value, significant nutritional contributions, and a remarkable capacity to enhance soil fertility. Despite these attributes, its field productivity often needs to improve, with the water deficit emerging as a primary hindrance. Consequently, genetic enhancements have been incorporated into select varieties, conferring tolerance to specific levels of water scarcity stress. This study aimed to elucidate the distinctions in protein expression patterns responding to water deficiency stress across nine bean varieties. Protein patterns were scrutinized through two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and selected protein spots were subjected to mass spectrometry analysis (MALDI-TOF MS/RP-LC-MS/MS). A comprehensive identification of 111 proteins was achieved and categorized based on their respective functions. Noteworthy among these were the desiccation-protectant protein (LEA14) and Desiccation-related protein PCC13–62, identified as proteins associated with the response to abiotic stress, particularly prevalent in the INIAP_473 cultivar. These findings underscore the potential for targeted genetic improvements to mitigate the impact of water deficit stress on common bean cultivation, contributing to enhanced agricultural resilience and productivity. • Water Deficit Challenge: Productivity is often limited by water deficit, posing a major agricultural challenge. • Protein Analysis: Analyzed protein expression in nine bean varieties using 2D-PAGE and MALDI-TOF MS techniques . • Abiotic Stress Response: Highlighted the role of these proteins in abiotic stress responses. • Potential Applications: Findings suggest potential use in genetic improvements to enhance water deficit tolerance. • Agricultural Impact: Aims to enhance resilience and productivity in bean cultivation by mitigating water scarcity effects.